![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K101005:Design
Designed by: Ellen Martin Group: iGEM08_Minnesota (2008-07-28)
Construct with LacI/LAMBDAcI promoter, RBS, GFP, Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 787
Design Notes
Majority of design considerations were dealt with during the design of the dually repressed promoter (K101001). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.
Source
This construct is composed of: LacI promoter ( R0010), LAMBDAcI promoter (R0051), RBS (B0032), GFP (E0040), and Terminator (B1006).